Dr Filippo Prischi
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Email
fprischi@essex.ac.uk -
Telephone
+44 (0) 1206 873370
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Location
3SW.5.03, Colchester Campus
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Academic support hours
Open door policy
Profile
Biography
My main research interest is the characterisation of protein complexes that are part of signalling pathways, in normal and cancer cells. My goal is to understand how the molecular machines that compose signalling pathways work together to transfer information. In my group, we use a cutting-edge multi-disciplinary approach that combines biophysical, enzymatic and structural studies. We use X-ray crystallography, Nuclear Magnetic Resonance and Electron Microscopy to visualise macromolecules at atomic and near atomic resolution. At this scale we are able to dissect cellular processes at a molecular level and describe the details at the base of cancer formation or development, thereby providing a solid base for medical advance. The main focus of our research is the study of phosphorylation cascades and protein kinases.
Qualifications
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PhD University of Siena and National Institute for Medical Research (MRC), (2010)
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M.Sc. Molecular Biology (110 summa cum laude) University of Siena, (2005)
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B.Sc. Biological Sciences (110 summa cum laude) University of Siena, (2003)
Appointments
糖心Vlog
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Senior Lecturer, Randall Centre for Cell and Molecular Biophysics, King's College London (3/4/2023 - present)
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Visiting Fellow, School of Life Sciences, 糖心Vlog (3/4/2023 - present)
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Senior Lecturer, School of Life Sciences, 糖心Vlog (1/10/2020 - 31/3/2023)
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Director of Recruitment, School of Life Sciences, 糖心Vlog (1/9/2021 - 1/10/2022)
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Deputy Director of Recruitment, School of Life Sciences, 糖心Vlog (4/11/2019 - 31/8/2021)
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Lecturer, School of Life Sciences, 糖心Vlog (1/6/2015 - 1/10/2020)
Other academic
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Research Associate, Imperial College London (1/5/2010 - 30/4/2015)
Research and professional activities
Research interests
Signalling pathways in lung and breast cancer
Drug Discovery
Protein Phosphoregulation
Structural Biology
Ribosomal Kinases pathways
Current research
Targeting RSK4 prevents both chemoresistance and metastasis in lung and bladder cancer
Understanding how RSKs regulate Transcription Factors in Triple Negative Breast Cancer
The role of 90kDa Ribosomal S6 Kinases in Steroid signalling
Flipping the switch; regulating protein synthesis in response to stress
Conferences and presentations
AtS6K2 regulates antioxidant capacity in response to salt and cold stress
88th Harden Conference - Beyond catalysis: kinases and pseudokinases 2022, Chesford Grange, Kenilworth, 2/11/2022
Targeting RSK4 prevents both chemoresistance and metastasis in lung and bladder cancer
Invited presentation, BCA/BACG Spring Meeting, 30/3/2021
Targeting RSK4 prevents both chemoresistance and metastasis in lung and bladder cancer
FEBS Congress 2019, Krakow, Poland, 10/7/2019
Targeting hnRNPA1 for the treatment of Small Cell Lung Cancer
Invited presentation, 6th EEMaX Symposium, Norwich, United Kingdom, 12/12/2018
Purification of protein kinases for structural studies
Invited presentation, AKTA user day, Cambridge, Cambridge, United Kingdom, 21/9/2018
The black sheep of the p90 ribosomal kinase family: RSK4
Invited presentation, 5th EEMaX Symposium, 5th EEMaX Symposium, Colchester, United Kingdom, 20/12/2016
Interaction of BiP and ER stress transducers is disrupted by unfolded proteins causing UPR activation.
16th International MST Meeting, 16th International MST Meeting, Munich, Germany, 18/6/2015
Phosphoregulation of human Ire1 RNase splicing activity.
CSB Open Day, CSB Open Day, London, United Kingdom, 6/6/2014
Phosphoregulation of human Ire1 RNase splicing activity.
Molecular Chaperones and Stress Responses, Molecular Chaperones and Stress Responses, Cold Spring Harbor, United States, 5/2014
Biochemical and Biophysical characterisation of c-IRE1 alpha
OPPF-MPL HTP Protein Production and Crystallization, Harwell, United Kingdom, 4/2011
Insights into the structure of an IscS/IscU/CyaY complex.
Invited presentation, Frataxin meeting, London, United Kingdom, 5/2010
Understanding the iron-sulphur cluster machinery: Characterization of the E. coli IscS/IscU complex.
IIX CCPN meeting, Penrith, United Kingdom, 8/2008
Key features of EF-HAND superfamily structure using paramagnetic probe.
XXXVII National NMR Congress, Verbania Pallanza, Italy, 9/2007
Dimerization of alpha-bungarotoxin monitored by paramagnetic probes: a new approach for protein-protein interaction studies.
Invited presentation, XXXVI National NMR Congress, Vietri Sul Mare, Italy, 9/2006
Teaching and supervision
Previous supervision
Degree subject: Biochemistry
Degree type: Doctor of Philosophy
Awarded date: 30/10/2023
Degree subject: Biochemistry
Degree type: Doctor of Philosophy
Awarded date: 21/8/2023
Degree subject: Cell and Molecular Biology
Degree type: Doctor of Philosophy
Awarded date: 20/4/2022
Degree subject: Biological Sciences
Degree type: Master of Science (by Dissertation)
Awarded date: 4/11/2021
Degree subject: Biological Sciences
Degree type: Master of Science (by Dissertation)
Awarded date: 1/7/2021
Degree subject: Biological Sciences
Degree type: Master of Science (by Dissertation)
Awarded date: 21/6/2021
Degree subject: Biological Sciences
Degree type: Doctor of Philosophy
Awarded date: 3/7/2020
Degree subject: Biochemistry
Degree type: Master of Science (by Dissertation)
Awarded date: 28/6/2019
Publications
Publications (3)
Cronin, R., Azadova, A., Marco, A., Laissue, PP., Brooke, GN. and Prischi, F., (2024). The p90 Ribosomal S6 Kinases 2 and 4 promote Prostate Cancer cell proliferation in androgen-dependent and independent ways
Roucairol, M., Georgiou, A., Cazenave, T., Prischi, F. and Pardo, OE., (2024). DrugSynthMC: an atom based generation of drug-like molecules with Monte Carlo Search
Dunnett, L., Das, S., Venditti, V. and Prischi, F., (2024). Enhanced identification of small molecules binding to hnRNPA1 via cryptic pockets mapping coupled with X-Ray fragment screening
Journal articles (37)
Dunnett, L., Das, S., Venditti, V. and Prischi, F., (2025). Enhanced identification of small molecules binding to hnRNPA1 via cryptic pockets mapping coupled with X-ray fragment screening. Journal of Biological Chemistry. 301 (4), 108335-108335
Roucairol, M., Georgiou, A., Cazenave, T., Prischi, F. and Pardo, OE., (2024). DrugSynthMC: An Atom-Based Generation of Drug-like Molecules with Monte Carlo Search. Journal of Chemical Information and Modeling. 64 (18), 7097-7107
Prischi, F., Jarvis, J. and Conte, MR., (2024). Meeting reports. The Biochemist. 46 (4), 45-45
Prischi, F., Birrell, JA., Carroni, M. and Worrall, J., (2024). Meeting Reports. The Biochemist. 46 (5), 50-50
Barbera, S., Raucci, L., Tassone, G., Tinti, L., Prischi, F., Santucci, A., Mongiat, M., Tosi, GM., Galvagni, F., Dimberg, A., Pozzi, C. and Orlandini, M., (2023). . International Journal of Biological Macromolecules. 224, 453-464
Hough, MA., Prischi, F. and Worrall, JAR., (2023). Perspective: Structure determination of protein-ligand complexes at room temperature using X-ray diffraction approaches. Frontiers in Molecular Biosciences. 10, 1113762-
Trampari, E., Prischi, F., Vargiu, A., Abi-Assaf, J., Bavro, V. and Webber, M., (2023). . npj Antimicrobials and Resistance. 1 (1), 2-
Corrales-Guerrero, L., Prischi, F. and D铆az-Moreno, I., (2023). Editorial: Weak interactions in molecular machinery volume II. Frontiers in Molecular Biosciences. 10, 1284353-
Galderisi, S., Milella, MS., Rossi, M., Cicaloni, V., Rossi, R., Giustarini, D., Spiga, O., Tinti, L., Salvini, L., Tinti, C., Braconi, D., Millucci, L., Lupetti, P., Prischi, F., Bernardini, G. and Santucci, A., (2022). . Archives of Biochemistry and Biophysics. 717, 109137-109137
Obomighie, I., Lapenas, K., Murphy, BE., Bowles, AMC., Bechtold, U. and Prischi, F., (2021). . Frontiers in Molecular Biosciences. 8, 636560-
Deganutti, G., Prischi, F. and Reynolds, CA., (2021). . Journal of Computer-Aided Molecular Design. 35 (2), 195-207
Prischi, F. and Filippakopoulos, P., (2021). . Frontiers in Molecular Biosciences. 8, 641932-
Cronin, R., Brooke, GN. and Prischi, F., (2021). . Oncogene. 40 (22), 3775-3785
Chrysostomou, S., Roy, R., Prischi, F., Thamlikitkul, L., Chapman, KL., Mufti, U., Peach, R., Ding, L., Hancock, D., Moore, C., Molina-Arcas, M., Mauri, F., Pinato, DJ., Abrahams, JM., Ottaviani, S., Castellano, L., Giamas, G., Pascoe, J., Moonamale, D., Pirrie, S., Gaunt, C., Billingham, L., Steven, NM., Cullen, M., Hrouda, D., Winkler, M., Post, J., Cohen, P., Salpeter, SJ., Bar, V., Zundelevich, A., Golan, S., Leibovici, D., Lara, R., Klug, DR., Yaliraki, SN., Barahona, M., Wang, Y., Downward, J., Skehel, JM., Ali, MMU., Seckl, MJ. and Pardo, OE., (2021). . Science Translational Medicine. 13 (602), eaba4627-
Trezza, A., Iovinelli, D., Santucci, A., Prischi, F. and Spiga, O., (2020). . Scientific Reports. 10 (1), 13866-
Brooke, GN. and Prischi, F., (2020). . Scientific Reports. 10 (1), 15917-
Spiga, O., Cicaloni, V., Fiorini, C., Trezza, A., Visibelli, A., Millucci, L., Bernardini, G., Bernini, A., Marzocchi, B., Braconi, D., Prischi, F. and Santucci, A., (2020). . Orphanet Journal of Rare Diseases. 15 (1), 46-
Zabet, NR., Catoni, M., Prischi, F. and Paszkowski, J., (2017). . Nucleic Acids Research. 45 (7), gkw1330-gkw1330
Prischi, F. and Pastore, A., (2017). . Frontiers in Molecular Biosciences. 4 (12), 12-
Prischi, F. and Pastore, A., (2016). . ADVANCED TECHNOLOGIES FOR PROTEIN COMPLEX PRODUCTION AND CHARACTERIZATION. 896, 351-368
Carrara, M., Prischi, F., Nowak, PR., Kopp, MC. and Ali, MMU., (2015). . eLife. 4 (4)
Popovic, M., Sanfelice, D., Pastore, C., Prischi, F., Temussi, PA. and Pastore, A., (2015). . Protein Science. 24 (6), 996-1003
Carrara, M., Prischi, F., Nowak, PR. and Ali, MMU., (2015). . The EMBO Journal. 34 (11), 1589-1600
Prischi, F., Nowak, PR., Carrara, M. and Ali, MMU., (2014). . Nature Communications. 5 (1), 3554-
Carrara, M., Prischi, F. and Ali, M., (2013). . International Journal of Molecular Sciences. 14 (3), 6454-6466
Bernini, A., Spiga, O., Venditti, V., Prischi, F., Botta, M., Croce, G., Tong, AP-L., Wong, W-T. and Niccolai, N., (2012). . Journal of Inorganic Biochemistry. 112, 25-31
Prischi, F., Konarev, PV., Iannuzzi, C., Pastore, C., Adinolfi, S., Martin, SR., Svergun, DI. and Pastore, A., (2010). . Nature Communications. 1 (1), 95-
Prischi, F., Pastore, C., Carroni, M., Iannuzzi, C., Adinolfi, S., Temussi, P. and Pastore, A., (2010). . Protein Expression and Purification. 73 (2), 161-166
Prischi, F., Giannini, C., Adinolfi, S. and Pastore, A., (2009). . The FEBS Journal. 276 (22), 6669-6676
Adinolfi, S., Iannuzzi, C., Prischi, F., Pastore, C., Iametti, S., Martin, SR., Bonomi, F. and Pastore, A., (2009). . Nature Structural & Molecular Biology. 16 (4), 390-396
Bernini, A., Venditti, V., Spiga, O., Ciutti, A., Prischi, F., Consonni, R., Zetta, L., Arosio, I., Fusi, P., Guagliardi, A. and Niccolai, N., (2008). . Biophysical Chemistry. 137 (2-3), 71-75
Venditti, V., Bernini, A., De Simone, A., Spiga, O., Prischi, F. and Niccolai, N., (2007). . Biochemical and Biophysical Research Communications. 356 (1), 114-117
Bernini, A., Spiga, O., Venditti, V., Prischi, F., Bracci, L., Tong, AP-L., Wong, W-T. and Niccolai, N., (2006). . Journal of the American Chemical Society. 128 (29), 9290-9291
Spiga, O., Padula, MG., Scarselli, M., Ciutti, A., Bernini, A., Venditti, V., Prischi, F., Falciani, C., Lozzi, L., Bracci, L., Valensin, PE., Caudai, C. and Niccolai, N., (2006). . Antiviral Therapy. 11 (7), 917-922
Bernini, A., Spiga, O., Venditti, V., Prischi, F., Bracci, L., Huang, J., Tanner, JA. and Niccolai, N., (2006). . Biochemical and Biophysical Research Communications. 343 (4), 1101-1104
Bernini, A., Spiga, O., Ciutti, A., Venditti, V., Prischi, F., Governatori, M., Bracci, L., Lelli, B., Pileri, S., Botta, M., Barge, A., Laschi, F. and Niccolai, N., (2006). . Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics. 1764 (5), 856-862
Spiga, O., Bernini, A., Ciutti, A., Chiellini, S., Menciassi, N., Finetti, F., Causarono, V., Anselmi, F., Prischi, F. and Niccolai, N., (2003). . Biochemical and Biophysical Research Communications. 310 (1), 78-83
Conferences (2)
Prischi, F., Chrysostomou, S., Roy, R., Chapman, K., Mufti, U., Peach, R., Ding, L., Mauri, F., Bellezza, G., Cagini, L., Barbareschi, M., Ferrero, S., Abrahams, J., Ottaviani, S., Castellano, L., Giamas, G., Pascoe, J., Moonamale, D., Billingham, L., Cullen, M., Hrouda, D., Winkler, M., Klug, D., Yaliraki, S., Barahona, M., Wang, Y., Ali, M., Seckl, M. and Pardo, O., (2019). Targeting RSK4 prevents both chemoresistance and metastasis in lung and bladder cancer
Chrysostomou, S., Roy, R., Prischi, F., Chapman, K., Mufti, U., Mauri, F., Bellezza, G., Abrahams, J., Ottaviani, S., Castellano, L., Giamas, G., Hrouda, D., Winkler, M., Klug, D., Yaliraki, S., Barahona, M., Wang, Y., Ali, M., Seckl, M. and Pardo, O., (2019). Targeting RSK4 prevents both chemoresistance and metastasis in lung cancer
Reports and Papers (5)
Deganutti, G., Prischi, F. and Reynolds, CA., (2020). Supervised molecular dynamics for exploring the druggability of the SARS-CoV-2 spike protein
Trezza, A., Iovinelli, D., Prischi, F., Santucci, A. and Spiga, O., (2020). An integrated drug repurposing strategy for the rapid identification of potential SARS-CoV-2 viral inhibitors
Brooke, GN. and Prischi, F., (2020). Structural and functional modelling of SARS-CoV-2 entry in animal models
Trampari, E., Holden, ER., Wickham, GJ., Ravi, A., Prischi, F., de Oliveira Martins, L., Savva, GM., Bavro, VN. and Webber, MA., (2019).
Bernini, A., Venditti, V., Spiga, O., Prischi, F., Botta, M., Tong, AP-L., Wong, W-T. and Niccola, N., (2011). The surface accessibility of 伪-bungarotoxin monitored by a novel paramagnetic probe
Dataset (11)
Spiga, O., Bernini, A., Ciutti, A., Chiellini, S., Prischi, F., Venditti, V. and Niccolai, N., Theoretical model of H4 influenza virus
Spiga, O., Bernini, A., Ciutti, A., Chiellini, S., Prischi, F., Venditti, V. and Niccolai, N., Human Influenza B virus trimer structure
Spiga, O., Bernini, A., Ciutti, A., Chiellini, S., Prischi, F., Venditti, V. and Niccolai, N., H5 influenza trimer structure
SPIGA, O., BERNINI, A., CIUTTI, A., CHIELLINI, S., Menciassi, N., Finetti, F., Causarono, V., Anselmi, F., PRISCHI, F. and NICCOLAI, N., S2 SUBUNIT MODEL OF SARS CORONAVIRUS S PROTEIN
Spiga, O., Bernini, A., Prischi, F., Venditti, V., Huang, JD. and Niccolai, N., Theoretical model of SARS Coronavirus helicase
Spiga, O., Bernini, A., Ciutti, A., Chiellini, S., Prischi, F. and Niccolai, N., S1 subunit model of SARS coronavirus S protein
Carrara, M., Prischi, F. and Ali, MMU., Crystal structures reveal transient PERK luminal domain tetramerization in ER stress signaling
Carrara, M., Prischi, F. and Ali, MMU., Crystal structures reveal transient PERK luminal domain tetramerization in ER stress signaling
Prischi, F. and Ali, MM., RSK4 N-terminal Kinase Domain in complex with AMP-PNP
Prischi, F. and Ali, MM., Phosphorylated RSK4 N-terminal Kinase Domain in complex with AMP-PNP
Prischi, F. and Ali, MM., RSK4 N-terminal Kinase Domain S232E in complex with AMP-PNP
Grants and funding
2019
Development of novel cancer treatments: structural studies of Nectin 4 in complex with bespoke anti-cancer peptides
Bicycle Therapeutics
Development of specific inhibitors that prevent hnRNPA1-mediated therapy resistance in small cell lung cancer.
糖心Vlog (EIRA/Research England)
2018
Flipping the switch; regulating protein synthesis in response to stress
Leverhulme Trust
2016
Phosphoregulation of Transcription Factors in Triple Negative Breast Cancer
Wellcome Trust
Contact
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